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Protein expression was induced in Triplusia ni Hi5 cells by infecting a 600 mL starting culture (1.0 10 6 cells/mL) in a 3 l flask with 100 to 400 L of V1 viruses, depending on its quality and age

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The weight loss effect depends specifically on AMY1R and AMY3R activation, as confirmed by a study in eBioMedicine that showed cagrilintide lowers body weight through these specific receptor subtypes in the brainstem