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After incubation, the contents of the wells were decanted into a discard container and washed with saline then emptied by flicking the plate gently

The pathogenicity of the SsCm1 mutants on different hosts was significantly reduced compared with the UF-1 and the complemented strain, whereas no significant variations among these strains were detected in colony morphology, hyphal growth rate, compound appressorium formation, sclerotium development, OA production, and content of cell wall hydrolase (Fig