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T7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter
Storage Buffer: 50% glycerol containing 25 mM sodium acetate (pH 4
The highly versatile TransforMax™ EC100™ E
100 mM sodium chloride
10 mM EDTA
E. cloni-10G CLASSIC Electrocompetent Cells, 48 rxns (SixPacks) cell projects T7 RNA Polymerase catalyzes theTable 1. E. cloni Competent Cells for Cloning and Library Construction E. cloni Cell Lines Transformation Efficiency (cfu g pUC DNA) Cloning Methylated DNA BAC, Cosmid Cloning Blue White Screening 10G SUPREME Electrocompetent >4 1010 YES YES YES without IPTG induction 10G ELITE Electrocompetent >2 1010 YES NO YES without IPTG induction 10G CLASSIC Electrocompetent >5 109 YES NO YES without IPTG induction 10GF ELITE Electrocompetent >2 1010 YES NO YES