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In addition to the targeted use of nicotine as a pesticide or as an ecological means of combating plant pests with tobacco brew (see, in German, Nicotine from Tobacco a Natural Method Against Plant Pests?), quantities of nicotine found can also stem naturally from the plant itself, or be the result of contamination from tobacco dust or smokers hands

These smokers [who light up first thing in the morning] have higher levels of nicotine and possibly other tobacco toxins in their body, and they may be more addicted than smokers who refrain from smoking for a half hour or more, said Dr