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Overcoming barriers such as staff shortages, limited resources, and cultural sensitivities is crucial for the long-term success and scalability of SBSPIs in fragile contexts

In the last hundred years nicotine reached peak popularity, as tobacco use was glamorised through cigarettes, and then equally swiftly fell from favour, as their addictive nature and danger to health became apparent

Tobacco mosaic hosts In addition to tomatoes and tobacco, the tobacco mosaic virus infects over 350 plant species, including cucumbers and all plants in the nightshade family, as well as many flowers and ornamental plants

The American Cancer Society recently highlighted the role of our flagship smoke-free product in the rapid decline in cigarette sales in Japan, which has seen its rate of decline in cigarette sales quintuple from a 2 percent decline per year from 2011 through 2015 to a 10 percent decline per year from 2015 through 2018, all without a major change in national tobacco control policy

When the log copy numbers were plotted against mean Cq, the efficiency was 96.73% and the correlation coefficient, R 2 , was 0.99 (Fig