Primary antibodies used and their concentrations are as follow: mouse IgG1 anti- catenin (1:400, BD, Franklin Lakes, New Jersey), mouse IgG2b anti-acetylated tublin (AcT) (1:10,000, Sigma, St Louis, MO), mouse IgG1 anti-TMPRSS2 (1:25, EMD Millipore, Temecula, CA), rabbit anti-ACE2 (1:50, Abcam, Cambridge, MA)
This measure should be interpreted as a broad indicator of social media use frequency, which did not capture platform-specific use, tobacco-related content exposure, or level of engagement
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