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This small molecule compound represents a selective inhibitor of NNMT, an enzyme that methylates nicotinamide (a form of vitamin B3) to produce 1-methylnicotinamide (1-MNA)

Wipe the rubber stoppers with alcohol and let them dry before each pierce, use a fresh sterile needle and syringe every time, and work in a clean preparation area

Treatment with 5-amino-1mq upregulates proliferationrelated genes (Ki67, PCNA), promotes cell cycle progression, and improves cell fusion efficiency to form more multinucleated myotubes, achieving functional levels similar to those of young mice.Additionally, 5-amino-1mq inhibits muscle stem cell apoptosis, reduces oxidative stress damage, and maintains stemness