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Typical protocols involve 2-4 sprays per nostril, 2-3 times daily, depending on your concentration and target dose

BMC Psychiatry 21 , 157 (2021)

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Yes, for dilution arithmetic

To account for the induction of ALT in the breast cancer cells (21NT and BT474) and non-induction in normal cells (IBR3 and HMEC), it is known that histone H1 is expressed at higher physiological levels in normal tissues and lower levels in breast cancer cells (Scaffidi 2016)