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Betaine is utilised in most tissues as an osmoproctectant in addition to its primary role as a methyl group donor in liver metabolism (reviewed by Zhao et al, 2018)

A PBS buffer (pH 7.3) is prepared by dissolving 805.5 mg of sodium chloride (Fisher Scientific), 606 mg of sodium phosphate dibasic anhydrous (Fisher Scientific), 247 mg of sodium phosphate monobasic anhydrous (Fisher Scientific), then QS to 200 g with sterile filtered DI water

Pseudomonas aeruginosa pyocyanin is critical for lung infection in mice

On the emulsifier side, it keeps your formula smooth and well-mixed by playing peacekeeper for ingredients that don't naturally get along (like oil and water)