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Cell 169.1 (2017): 132-147.e16

Controla grasa efectiva

This was done either before or after the Ravitch procedure

Understanding these methodological differences helps researchers select appropriate protocols for their experimental goals (Pickart et al., 2015)

Primary mouse neuron culture For primary mouse neuron cultures, #1.5 acid-washed glass coverslips were added to a 12-well culture plate, coated with 1 ml per well of a 50 g Poly-D-Lysine (Gibco, Cat# A3890401) and 2 g/mL laminin solution in sterile diH 2 O, put in a 37 C water-jacketed incubator for 4872 h, rinsed twice with sterile diH 2 O, and allowed to dry prior to cell plating