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The Real-Time PCR was performed with a CFX ConnectTM Real-Time PCR Detection System (BIO-RAD, Warsaw, Poland) and following cycling conditions: 95 C for 2 min, followed by 40 cycles at 95 C for 15 s, annealing for 15 s, and elongation at 72 C for 15 s

Look for the exact compound name plus an HPLC trace and a mass spec (LC-MS) result that matches it

This depends on how severe the deficiency is, any health conditions, and how well the therapy works