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Given that the Phase I clinical trial in IPF demonstrated a reduction of senescent cells and SASP within eleven days after a single treatment of D+Q, and improvements in physiological outcomes after 10 weeks (105), we reasoned that a three-month period would provide important feasibility, compliance and efficacy data, as well as potentially reveal other cognitive and biomarker effects useful for designing a larger study

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Briefly cells were homogenized in lysis buffer and incubated with lucigenin (5 mol/L, Sigma-Aldrich) and NADPH (0.1 mmol/L

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