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Pituitary primordia were explanted from 11-day-old rat fetuses and cultured in a synthetic medium enriched with either DSIP at several concentrations, GnRH (10(-9) M) or TRH (10(-9) M)

By focusing on metabolic pathways at the cellular level, it provides an advanced method for improving athletic performance, decreasing fat accumulation, and maybe prolonging lifespan

The cells were washed once in with 50 L PBS (Gibco, #14190-094) after which 30 L of each concentration from the dilution series was added to the 384-well assay plate with cells

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Temperatures above 30C begin causing degradation