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J Nutr Biochem 25:702709 Fangbo X, Yu Z, Mengqiu L, Qi C, Yonghong L, Xinmin L, Ruile P (2015) Antioxidant and anti-fatigue constituents of okra

After the transfection was completed in the cells, the cells were collected and lysed using RIPA (Radio Immunoprecipitation Assay), after which 2 x SDS PAGE (polyacrylamide gel electrophoresis) loading buffer was added to the cell samples, mixed thoroughly and then boiled in a water bath for 15 min

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doi: 10.1016/j.molmet.2018.02.006

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