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Samples were diluted with 50 mM Tris-HCl to bring urea concentration down to below 2 M to prevent trypsin inhibition

Puerarin attenuates LPS-induced inflammatory injury in gastric epithelial cells by repressing NLRP3 inflammasome-mediated apoptosis

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Research has shown that even in people without any underlying health conditions, when taking a 500 microgram oral supplement, only 10 micrograms (or 2%) is absorbed (Carmel, 2008)

Furthermore, the injection of nanoscale iron into the cortex induces chronic epilepsy in mice and simulates brain injury due to micro-hemorrhage, which in turn, gives rise to different degrees of spontaneous epileptiform events (Mori et al., 1990)