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[53] Lin YW, Hu ZH, Wang X, et al

RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR

Studies in rats and beagle dogs have shown a rapid elimination half-life of less than 30 minutes after intravenous administration

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