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Briefly, cells were fixed for 15 min at RT with 1% formaldehyde

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Downstream migration is mediated by self-secreted ligands, specifically CCL19 and CCL21, which bind to the chemokine receptor CCR7 [233]

Together they target both the structural immune deficit and its downstream consequences

In the Methods, we explain why we take the multiplier, S , of the V max values of GCL and GPX to have the following dependence on estradiol, E 2: Thus, since male E 2 is 0.09 nM [26] , S = 1 for the male