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Of note, the commercial Iscoves Modified Dulbeccos Medium (IMDM) and Serum Free Expansion Medium (SFEM), which were used as the basal medium for erythroblast cultures, contain a supraphysiological concentration of cystine at 292 M as compared to physiological plasma concentration of 4075 M cysteine in SCD patients, which decreased further to 3060 M during vaso-occlusive pain episodes 36

Funding This work was supported by the German Research Foundation (DFG) via the SFB/TRR205 (KF, SRB, AE-A, WT), the IRTG-2251/2 (NB, MT, KF, SRB, AE-A, WT), the IRTG-3019/1 (SRB, WT), and the Else Krner Fresenius-Stiftung (WT, 2025_EKES.07)

Other cells were provided by co-authors or collaborators (SW480 and HT29 were provided by McDade and Longley (QUB)

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