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Other purification options
a simple nickel column purification is sufficient for endotoxic shock response negative recombinant proteins suitable or downstream eukaryotic cellular response assays
Unit Definition: One unit of CircLigase enzyme converts 1 pmol of a linear 5´-phosphorylated CircLigase Standard 55-mer Oligo into an exonuclease I-resistant circular form in 1 hour at 60°C under standard assay conditions
composed of 200 mM Tris-HCl pH 8
5 kb gene (plasmid DNA)
Xylene DGF Other purification options
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