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If activity ramps up based on symptom changes alone, reinjury becomes likely

Preparation of 1 Biotinylated-Conjugate and 1 Streptavidin-HRP Working Solution: Briefly spin or centrifuge the stock solutions of Biotinylated-Conjugate and Streptavidin-HRP to ensure they are well mixed

These changes culminated in a marked 30% decline in left ventricle ejection fraction by day 52, confirming substantial Dox-induced systolic dysfunction

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