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10.1073/pnas.0504588102 24 DalekeD

Cycling conditions consisted of an initial denaturation step at 95 C for 3 min, followed by 40 cycles at 95 C for 30 s, 60 C for 30 s, and 72 C for 30 s, and then 72 C for 10 min

Notice the copper amount changes sharply between the two styles

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Our results show that glutathione efflux from hepatocytes results in high local concentrations of both glutathione and cysteine in liver sinusoids, which transforms the nanoparticle surface chemistry, reduces its affinity to serum protein and significantly alters its blood retention, targeting and clearance