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10.1016/j.taap.2004.09.008 Toxicol Appl Pharmacol

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Ran activation assay Levels of RanGTP were assessed by immunoprecipitation using RanBP1(RBD)-GST fusion proteins bound to glutathione-sepharose beads, which selectively isolate the active form of Ran (RanGTP) in the lysis Buffer: 25 mM HEPES, Ph 7.5, 150 mM NaCl, 1% NP-40, 10 mM MgCl2, 1 mM EDTA, 2% Glycerol, 10 mM NaF, protease inhibitor tablet (Roche), followed by western blot analysis with anti-Ran antibody

For individuals living with Long COVID, myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), dysautonomia, and mast cell activation syndrome (MCAS), the search for answers often leads to the microscopic world of cellular metabolism