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The assay uses two chemicals, 6 hydroxydopamine (6HD) and diethylenetriaminepentaacetic acid (DETAPAC), to generate anions, which are reduced in the presence of SOD
The bound protein was eluted using 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 0.01% (w/v) LMNG and 0.0006% (w/v) CHS, 1 M peptide, and 5 mM EGTA and 0.1 mg/mL Flag peptide
Efficacy and safety of tirzepatide, dual GLP-1/GIP receptor agonists, in the management of type 2 diabetes: a systematic review and meta-analysis of randomized controlled trials
doi: 10.1007/s00401-019-02049-1 30 WangSDengJFuHGuoZZhangLTangP
