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Deficiency is widespread, with peer-reviewed research describing it as a global health concern affecting populations across all age groups and geographies (Cashman et al., American Journal of Clinical Nutrition , 2016

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Lysis was induced by recombinant tPA (final concentration, 60 ng/mL, Boehringer Ingelheim, Ingelheim, Germany) and involved mixing citrated plasma with calcium chloride (final concentration, 15 mmol/L), human tissue factor (Innovin, Dade Behring, final concentration, 0.6 pmol/L ), and phospholipid vesicles (final concentration, 12 mol/L), all diluted in Tris-buffered saline

Nutrients 15:48