Preparation of working reagents KH 2 PO 4 (0.2 M) and Na 2 PO 4 (0.2 M) were mixed at a ratio of 40.5:9.5, (v/v), and the pH, if necessary, was adjusted to 7.4 by adding KH 2 PO 4 or Na 2 HPO 4 solutions drop-wise
How to use: Apply a thin layer to clean, dry skin at night, massage gently until absorbed, use sunscreen daily, and avoid mixing with stronger active ingredients to prevent irritation
Finally, the proteins were extracted and applied to WB analysis
In addition, the mechanism of detoxification by GSTD2 was revealed via its strong affinity towards isothiocyanate and catalysis of the conjugation between GSH and isothiocyanate
The hydroxyl radical is formed by the Fe/Cu-catalyzed decomposition of hydrogen peroxide (Eq