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In specific embodiments, auris formulations described herein are formulated to provide a delivered perilymph-suitable osmolarity at the target site of action of about 250 to about 320 mOsm/L and preferably about 270 to about 320 mOsm/L

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For the thickness of the retinal tissue, internal plexiform layer, and internal nuclear layer, the measurements (ISSA program (VAMSTEC, Zagreb, Croatia) from the area of maximal tissue damage detected using semi-serial sections, five high-power fields were randomly selected for analysis, were made with an ocular micrometer at 20 magnification within 0.5 mm from the optic disc