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3.2 Engineering RBC metabolism and storage conditions During their storage at refrigerated temperatures, red blood cells go through several biochemical and morphological modifications known as storage lesion. Indeed, during this period, 2,3-BPG and other high-energy phosphate compounds gradually decrease because of the reduced glycolytic activity at 1C6C storage temperatures, the acidification of the intracellular pH, and the reversible and irreversible oxidation of the active site of rate-limiting glycolytic enzymes, such as glyceraldehyde 3-phosphate dehydrogenase

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