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The elution was concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column that was pre-equilibrated with 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 1 M peptide, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS

LONP1 and arthritis Osteoarthritis (OA) OA is an age-related degenerative joint disease characterized by cartilage degradation, synovial inflammation, and subchondral bone remodeling [137]

Tissue healing and prolonged inflammation reduction can require weeks to months

Follow-up work has examined GHK-Cu's activity on matrix metalloproteinases, proteoglycan synthesis, and glycosaminoglycan production in the dermal extracellular matrix (Simeon et al., Life Sci, 2000