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Hearts were washed with cold (+4C) KCl 0.9% solution, weighed and homogenized in isolation buffer based on 0.1 M potassium phosphate buffer (KPE) with the addition of 5 mM EDTA (EDTA sodium salt) at pH = 7.5, 0.1% Triton X-100% and 0.6% sulfosalicylic acid

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The same McCoy 2013 paper reported that Dihexa reversed scopolamine-induced deficits and improved learning in aged rats in the Morris water maze again, animal data, from a paper now carrying an Expression of Concern

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IMPORTANT NOTES: This treatment is not suitable for you if you