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To indirectly measure NO levels from nitrite, nitrate is enzymatically reduced to nitrite using nitrate reductase, enabling the total nitrite amount to be determined (Glin 2012)

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Cells were washed and fixed with methanol (100%) and permeabilized with Triton X-100 (0.5%) in PBS

doi: 10.1152/ajpgi.00272.2005 50 MokhtarF

Given this, and also because of a very high inter-individual variability of TL in same-age individuals, longitudinal research of intra-individual rates of age-related decline in TL is likely a more useful design than the cross-sectional one in studying the relationship between TL and mortality