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After the first incubation, for the separation of the sexually mature stages of the parasite (zygotes), the blood suspension was centrifuged (400 x g, 5 min, and 36 C), and the pellet was resuspended with RPMI 1640 medium before purification with 45% Percoll (GE Healthcare)
NM_002084.3), in which the codons for Cys-12 (TGC), Cys-81 (TGC) and Cys-136 (TGT) were replaced with the codons for Ser (TCG) and the codon for Sec-53 (TGA) was changed to Cys codon (TGC)
10.3389/fcvm.2022.837371 133 SunZ.HanY.SongS.ChenT.HanY.LiuY
88 Despite differences in biochemical and pharmacological properties of the five currently approved TNFi, there does not seem to be a clinically meaningful difference between them in terms of efficacy and safety
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