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However, it had similar temporal pattern, and this activation was prevented by K252a kinase inhibitor and corresponding MEK1 and PI3 kinase inhibitors (PD98059 and LY294002 respectively)

Afterwards, membranes were stained with primary antibodies against pSTAT3 (1:1,000), STAT3 (1:1,000), GAPDH (1:10,000), Prx1 (1:1,000), Trx1 (1:1,000), and respective secondary antibodies

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Comprehensive evaluation of mitochondrial redox profile, calcium dynamics, membrane integrity and apoptosis markers in a preclinical model of severe penetrating traumatic brain injury

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