Serum limitation-induced senescent fibroblasts model and effect of FOXO4-DRI Passage 3 fibroblasts derived from keloids and normal skins were cultured in a Dulbeccos Modified Eagles medium (DMEM, Hyclone, Logan, Utah, USA) containing 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) to 90% confluence, and then cultured in a DMEM containing 2% FBS for 3 days to construct a serum limitation-induced senescent fibroblast model
[DOI] [PubMed] [Google Scholar] 80.El-Hafidi M., Franco M., Ramrez A.R., Sosa J.S., Flores J.A.P., Acosta O.L., Salgado M.C., Cardoso-Saldaa G
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Over that time we have developed more nonsurgical ways, including the use of regenerative medicine techniques in the office, to shorten the impact of frozen shoulders for our patients
