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mice Six to eight month old C57BL/6 mice were injected with 400,000 B16-F10 cells in 200 l PBS by tail vein injection and subsequently received daily intraperitoneal injections of either D-Nle-X-Cys-NH(CH 2 ) 5 CONH 2 (10 g/kg and 100 g/kg) or a PBS vehicle control

Mice received a regular rodent chow diet (2.99 kcal/g

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Patients who are pregnant or breastfeeding should talk to their doctor before starting peptide therapy

Unlike many peptides that act through a single well-defined receptor, GHK-Cu exerts its biological effects through multiple mechanisms including direct stimulation of collagen and glycosaminoglycan synthesis, activation of antioxidant enzymes, modulation of metalloproteinase activity, promotion of angiogenesis, and broad regulation of gene expression across hundreds of genes involved in tissue repair, inflammation, and cellular protection