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Protoplast Isolation and Transfection Two-week-old seedlings grown on 1/2 MS were used to isolate protoplasts as described previously with minor modifications ( Transcriptome Analysis Total RNA was extracted from 3-week-old plants with TRIzol (Invitrogen) and purified using a GeneJET plant RNA purification kit (Thermo Fisher Scientific)
Besides, the intrinsic instability of persulfides complicates the generation of reliable standards, and the stability of the persulfide alkylation products has been questioned
Brussels, BE
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The primary aim of this study is to elucidate the molecular mechanisms underlying CP-induced ovarian injury, with a focus on oxidative stress, inflammatory signaling, and apoptotic pathways, focusing on nuclear factor-kappa B (NF-B), nuclear factor erythroid-2-related factor 2/heme oxygenase-1 (Nrf2/HO-1), Toll-like receptor 4 (TLR4), nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome, silent information regulator-1 (SIRT1), and other signaling pathways involved in the pathogenesis of ovarian injury caused by CP
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