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2.3 Laboratory-scale glutathione affinity chromatography Unless otherwise indicated, laboratory-scale GSH affinity chromatography was performed with GSTPrep FF 16/10 columns packed with GSH Sepharose 4 FF resin (Cat#: 28936550, Cytiva) run at a 6 min residence time using an AKTA Pure 150 M FPLC system (Cytiva) with UNICORN system control software (Cytiva)

Refrigeration doesn't stop degradation entirely, but it slows it down to a crawl, preserving the peptide's integrity for the typical duration of a research protocol (usually 30-60 days post-reconstitution)

Thus, as an accessible antioxidant, the presence of an operative recycling system makes AsA an excellent buffer in the plant cell [11]