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It can also provide relief from symptoms of conditions like fatigue, dehydration, and vitamin deficiencies

Nucleofections were performed with a protocol optimized for MEFs using the Neon electroporation system (Invitrogen) (cells resuspended in Gibco 1xPBS, 1350 mV, 1 pulse, 30 ms) or Amaxa nucleofection system (Lonza) with proprietary nucleofection protocols optimized for HCT116 or MEFs, and cells resuspended in Solution I (5 mM KCl, 15 mM MgCl 2 , 50 mM Mannitol, 120 mM sodium phosphate solution pH 7.2)

doi: 10.1016/j.toxicon.2012.05.020 Yang, Shilong, Liu, Zhonghua, Xiao, Yao, Li, Yuan, Rong, Mingqiang, Liang, Songping, Yu, Haining, King, Glenn F., Lai, Ren and Zhang, Zhiye (2012)