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A 15mg vial reconstituted with 3mL yields 5mg/mL, where each 0.01mL increment represents 50mcgacceptable precision for most research protocols

Simultaneously, mean fluorescence intensity was determined at an unbroken region of the PM lacking membrane protrusions, and the ratio over total cell fluorescence was used to define relative PM content for each GFP-construct

This lets them fully penetrate before you seal everything in

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