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Samples were then incubated with 30 l of freshly made MOX solution (40 mg methoxyamine hydrochloride dissolved in 1ml of pyridine) for 45 min at 45C to protect keto groups, followed by the incubation with 70 l of MTBSTFA (N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide) for 45 min at 45C to derivatize metabolites

For example, ferumoxytol can selectively kill leukaemia cells with low FPN expression while exhibiting low toxicity to normal hematopoietic cells, demonstrating its potential for targeted leukaemia therapy [140]