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Materials and methods Cell culture and reagents CRC cell lines, HCT116, RKO, SW480, and LOVO, were obtained from the American Type Culture Collection (Manassas, VA) and authenticated by the manufacturer

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While compounds 6 - 8 caused maximal inhibition of TGR within 15 min (referred to here as fast inhibitors

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In a mouse model of diet-induced NASH, Notch signaling in hepatocytes induces osteopontin secretion, paracrine HSC activation and hepatic fibrosis, which are abrogated by Notch inhibition (132)