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The nuclear translocation of these two proteins could serve as a desensitization marker for GPCRs, such as D2-like receptors

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Using sulforaphane as the model isothiocyanate, it has indeed been established that its metabolites sulforaphane-glutathione, sulforaphane-cysteine-glycine, sulforaphane-cysteine, and sulforaphane N-acetylcysteine collectively known as dithiocarbamates ( Figure 2 ), are ultimately excreted in the urine (4)

Proteins that bound the antibody were visualized by chemiluminescence procedures (Clarity Western ECL substrate Bio-Rad)