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Model Selection: In vitro: Primary hepatocytes, adipocytes, cells with high NNMT expression In vivo: DIO models, aging models, genetic obesity models Consider NNMT expression levels in chosen model 5

Extended treatment continued to limit body weight gain and white adipose tissue mass, lowered total plasma cholesterol, and showed measurable accumulation in adipose tissue consistent with the compounds lipophilic profile

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In some tumor types, NNMT activity helps the tumor grow

These observations are consistent with incretin-related pathway activation patterns commonly seen in tri-agonist research