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The lysate was centrifuged at 13000 g at 4 C, and supernatant subjected to deproteinization procedure via TCA precipitation (Abcam

While vitamin B12 deficiency can contribute to hair thinning and hair loss, correcting the deficiency may help restore hair health in affected individuals

DOI: 10.1002/pat.5154

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After TMB substrate solution is added, only those wells that contain Human IGFBP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color