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PWL was defined as the time that it took the rat to exhibit pain-related behaviors, such as paw licking, hindlimb withdrawal, or jumping, obtained from three repeats of the experiment (5 min intervals) after being placed on the hot plate surface at 52 0.5C

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Welsh JW, Seyedin SN, Allen PK, Hofstetter WL, Ajani JA, Chang JY, Gomez DR, Amini A, Swisher SG , Blum MA, Younes AI, Nguyen QN, Minsky BD, Erasmus JJ, Lee JH, Bhutani M, Komaki RU

enterocytes), as well as for the phagocytic activity of macrophages and production of GSH, which is the most potent antioxidant in the body [20, 33, 35]