After three washes in PBS, sections were incubated for 3 h in blocking solution (3% bovine serum albumin (BSA), 0.3% Triton X-100 in TBS) and then for 24 h at 4 C with mouse monoclonal antibodies against tyrosine hydroxylase (TH) diluted in PBS containing 1% BSA
Song SO, Yun JS, Ko SH, et al
5B), which serves as a critical antioxidant component preserving intracellular redox balance and participating in extensive metabolisms [42, 44]
These effects are mediated through inhibition of the NF-B/NLRP3/caspase-1 and Bax/Bcl-2/caspase-3 pathways, alongside activation of Nrf2/HO-1, p-AMPK, and -Klotho signaling (Khallaf et al
Most higher-quality research on inositol focuses on oral supplementation , particularly in PCOS-related outcomes and metabolic markers