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Outliers were removed from control data after identification with ROUT Q = 0.5% (number of outliers removed: NAD + /NADH ratio n = 2, NADP + /NADPH ratio n = 11 and GSH/SSG ratio n = 6, no outliers were identified for individual metabolites)

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Longevity pathway activation NAD+ plays a crucial role in activating sirtuins, a family of proteins involved in cellular repair, stress response, and aging

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