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Human studies in placebo-controlled, small-scale, double-blind groups have also revealed the therapeutic impact of the probiotic product on DMT2

Then pellets were resuspended in lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280), thoroughly vortexed for 1 min and incubated for 15 min on ice

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Curr Pediatr Reviews 18(4):274285 Forny P et al (2021) Guidelines for the diagnosis and management of methylmalonic acidaemia and propionic acidaemia: first revision