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6 () : 10:00 - 23:00 ,

The following day, sections were rinsed with PBS and then incubated for 3 h at 2225 C with Cy3-conjugated anti-mouse (Jackson ImmunoResearch, 715-165-150) at 1:500 dilution in a solution of 1.5% BSA in PBS, respectively

Dane was also considered a sex symbol because of the role, with his character earning the nickname, "McSteamy." He played the role until he left the show in 2012, when Mark was killed off