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Therefore, the significance of PGAM5 in necroptosis requires further investigation and confirmation

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The level of intracellular glutathione is a key regulator for the induction of stress-activated signal transduction pathways including jun N-terminal protein kinases and p38 kinase by alkylating agents

The samples were subsequently washed with 1 ml of 1% BSA and 0.5% Tween-20 in PBS, centrifuged and the pellets were resuspended in 1 ml of PI solution (20 g ml 1 propidium iodide (Sigma-Aldrich, P4170), 0.5 mg ml 1 RNase A (Sigma-Aldrich, R5503) and 0.1% Triton X-100 in PBS)

Thus, GH injection mimicked the effects induced by chemogenetic activation of AgRP neurons 13