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The log-rank test did not demonstrate differences among the two curves ( p = 0.654) (Supplementary Figure S2)

Methylene blue aquaculture solutions benefits include broad spectrum antimicrobial action to protect aquatic life, has low toxicity levels when used at recommended concentrations and improves survival rates in hatcheries

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The ultrasound machine should be placed so that the clinician can look directly at the screen as well as the site of needle entry in the same line of sight (Pictures 2A and 2B)

Blinded experiments were conducted to assess histological analysis to avoid biases